Microbiology Test #1                                       NAME______________________________

Chapters 1-4&6

September 19, 2005

 

If any of you lacks wisdom, let him ask of God who gives to all liberally, but let him ask in faith.  James 1:5

 

 

There are eight levels of classification for all living things starting with “domain” and ending with “species,” list those in between. 0.5pts each

 

Domain

______________________________

______________________________

______________________________

______________________________

______________________________

______________________________

Species

 

 

1.                  Which domain contains procaryotes?

A)    Archaea

B)     Eukarya

C)    Bacteria

D)    Plantae

 

2.                  Which of the below is the correct writing of the scientific name for the famous orange bread mold that infected French bakeries?

A)    Neurospora crassa

B)     NEUROSPORA CRASSA

C)    Neurospora Crassa

D)    Neurospora crassa

E)     Neurospora Crassa

 

3.                  The term used to describe a microorganism that causes a disease is _________________.

A)    microbe

B)     bug

C)    bacteria

D)    virus

E)     pathogen

4.                  The microorganisms that do not have a nucleus in their cells are called:

A)    archaea

B)     procaryotes

C)    pathogens

D)    eucaryotes

E)     microbe

 

5.                  Which of the following is a reason to study microorganisms?

A)    They are decomposers of dead material

B)     They are the most abundant organisms on earth

C)    They provide nitrogen to us all

D)    They cause disease

E)     All of the above

 

6.                  Which of the following is not a process in the scientific method?

A)    belief in a preconceived idea

B)     formulate a hypothesis

C)    systematic observation

D)    laboratory experimentation

E)     development of a theory

 

7.                  Joe Scientist has made the discovery of a lifetime – a new cologne that smells like money – it should attract women like flies!  He wants to test its effects.  He decides to try his invention by putting it on immediately after a shower.  He will compare the response he gets from women with this method to the response he gets when he doesn’t shower and doesn’t wear the miracle cologne.  Is this a good experimental approach?  In one sentence tell me why or why not.

 

 

 

 

 

 

 

 

 

8.                  The subatomic particles that fly around the nucleus are:

A)    electrons

B)     protons

C)    neutrons

D)    protons and neutrons

E)     protons and electrons

 

9.                  Cations are

A)    charged subatomic particles

B)     atoms that have lost electrons

C)    radioactive isotopes

D)    atoms with extra neutrons

10.              Isotopes are atoms of the same element that differ in their:

A)    neutron number

B)     electron number

C)    proton number

D)    chemical properties

 

 

Write the name of the element that correlates with the correct description. 1pt each

Carbon                   oxygen                         nitrogen                        hydrogen

 

___________________ backbone of organic molecules

___________________ component of proteins and nucleic acids

___________________ component of water and all organic molecules

___________________ component of carbohydrates and water, needed for respiration

 

11.              The three main bond types include all EXCEPT:

A)    covalent

B)     molecular

C)    ionic

D)    hydrogen

 

12.              A solution of pH 7 compared to a solution of pH 9:

A)    is more basic

B)     has no OH- ions

C)    has more H+ ions

D)    has a higher pH

 

13.              Which of the following is NOT an amphipathic molecule?

A)    detergent

B)     phospholipid

C)    glucose

 

14.              All of the following are considered lipids except:

A)    cholesterol

B)     starch

C)    phospholipid

D)    triglyceride

 

15.              Label the parts of the nucleotide appropriately:

sugar        nitrogenous base        phosphate

 

 

 

 

 

 

Finish the rest of the table 8pts

 

Organic

Molecule

 

Monomer

 

Polymer

Bond type between monomers

 

 

sugar/monosaccharide

 

 

glycosidic

 

 

fatty acid & glycerol

 

di& tri glycerides

 

 

proteins

 

 

polypeptides

 

 

 

nucleotides

 

 

phosphodiester

 

 

16.              Which of the following would have glycosidic bonds?

A)    triglycerides

B)     saccharides

C)    polypeptides

D)    polysaccharides

E)     ATP

 

17.              What part of a phospholipid forms hydrophobic tails?

A)    fatty acids

B)     glycerol

C)    phosphate

D)    alcohol

 

18.              An amino acid is made of three repeating atoms N-C-C.  In the polypeptide below indicate where the variable side groups (R) would attach and where the peptide bonds would be

 

 

 

 N – C – C – N – C – C – N – C – C

 

 

 

 

 

 

19.              Knowing what you do about terms in biology, what could the imaginary word aminoglycology mean?

A)    The study of nucleic acids and proteins

B)     The study of proteins and lipids

C)    The study of proteins and carbohydrates

D)    The study of lipids and nucleic acids

20.              The Five I's of studying microorganisms include all of the following except:

A)    inoculation

B)     incubation

C)    infection

D)    isolation

E)     identification

 

21.              The term that refers to the purposeful addition of microorganisms into a nutrient medium is:

A)    isolation

B)     inoculation

C)    immunization

D)    infection

E)     contamination

 

22.              Which of the following characteristics refers to the microscope's ability to show two separate entities as separate and distinct?

A)    resolving power

B)     magnification

C)    refraction

 

23.              The type of microscope in which you would see brightly illuminated specimens against a black background is:

A)    bright- field

B)     dark-field

C)    phase-contrast

D)    fluorescence

E)     electron

 

24.              This microscope does not use light in forming the image:

A)    bright- field

B)     dark-field

C)    phase-contrast

D)    fluorescence

E)     electron

 

25.              This microscope is the most widely used and shows cells against a bright background:

A)    bright- field

B)     dark-field

C)    phase-contrast

D)    fluorescence

E)     electron

 

26.              Bacterial cells could have any of the following appendages except:

A)    flagella

B)     cilia

C)    fimbriae

D)    sex pili

 

27.              The short, numerous appendages used by some bacterial cells for adhering to surfaces are called:

A)    flagella

B)     cilia

C)    fimbriae

D)    sex pili

 

28.              The transfer of genetic material from one cell to another during bacterial conjugation involves rigid, tubular appendages called:

A)    flagella

B)     cilia

C)    fimbriae

D)    sex pili

 

29.              All bacterial cells have:

A)    DNA - genetic material

B)     fimbriae

C)    endospores

D)    capsules

E)     flagella

 

30.              The term that refers to the presence of flagella all over the cell surface is:

A)    amphitrichous

B)     lophotrichous

C)    monotrichous

D)    peritrichous

 

31.              The outcome of the Gram stain is based on differences in the cell's:

A)    ribosomes

B)     inclusions

C)    cell wall

D)    flagella

 

32.              The most immediate result of destruction of a cell's ribosomes would be:

A)    material would not be able to cross the cell membrane

B)     protein synthesis would stop

C)    destruction of the cell's DNA

D)    loss of capsule

 

33.              If bacteria living in salty seawater were displaced to a freshwater environment, the cell structure that would prevent the cells from rupturing is:

A)    cell wall

B)     cell membrane

C)    capsule

D)    slime layer

 

 

34.              There are three main bacterial shapes, quickly sketch and name them.  3pts

 

 

 

 

 

 

 

 

 

35.              Peptidoglycan is a unique macromolecule found in bacterial:

A)    cell membranes

B)     cell walls

C)    capsules

D)    slime layers

E)     inclusions

 

36.              The bacterial chromosome:

A)    is located in the cell membrane

B)     is in the nucleus

C)    is usually circular

D)    forms a linear strand of DNA

 

37.              The function of bacterial endospores is:

A)    enable to cell to adhere to surfaces

B)     increase the surface area of the cell membrane

C)    protection of genetic material during harsh conditions

D)    storage of excess cell materials

 

 

Protein Structure      primary 1o       secondary 2o       tertiary 3o       quartinary 4o    1pt each

 

_________________  is how the α helices and β sheets interact with each other

_________________  is multiple proteins interacting with each other

_________________  is the sequence of amino acids

_________________  is made of α helices, β sheets, or loops

 

 

38.              All of the following are processes that define life except:

A)    ability to invade host cells

B)     growth

C)    reproduction and heredity

D)    metabolism

E)     movement and/or response to environment

39.              Which of the following is a unique characteristic of viruses that distinguishes them from the other major groups of microorganisms?

A)    cause human disease

B)     lack a membrane enclosed nucleus

C)    cannot be seen without a microscope

D)    contain genetic material

E)     lack cell structure

 

 

40.              Which of the following is not found in all viruses:

A)    nucleic acid

B)     protein

C)    envelope

D)    capsid

 

 

 

 

EXTRA CREDIT 3pts

List the five steps in the cycle of bacteriaphage infection