BIOL220

Microbiology

4 credit hours

Southwestern Adventist University

Biology Department

Fall 2008

 

Class Time:    Monday, Wednesday, Friday 8:30 - 9:20 am     OR    1:00 – 1:50pm

Lab Time:      Wednesday 3 – 5:50pm OR Wednesday 6:30-9:20pm OR Thursday 6:30-9:20pm

 

Instructor:      Suzanne Phillips, Ph.D. 

Office: Scales Hall # 224B; office hours are posted on the door

Telephone: ext. 6274

E-mail:  suzannephillips@swau.edu

 

Textbooks:     Foundations in Microbiology 6th edition by Talaro McGraw-Hill publishers

Laboratory Exercises in Microbiology 7th edition by Harley McGraw-Hill publishers

 

Description:     Introduction to the structure, function and control of microorganisms in the environment with special emphasis on those organisms of medical importance. Introduction to immune responses and mechanisms.

 

General purpose of the course:

To acquire the root understanding of and appreciation for the functions and structures of microorganisms, the diseases they cause, and the human immune system and to be able to demonstrate this understanding in appropriate circumstances.

 

Specific objectives of the course:

                        To gain an understanding of:

Prokaryotic vs. Eukaryotic cells

Cell chemistry

Viruses

Microbial structure, function, and metabolism

Laboratory methods in microbiology

Immunology

Diseases caused by microbes and their control

 

General education requirements:

Microbiology satisfies part of SWAU’s general education requirements because it addresses many of the objectives and expected outcomes of the General Education program including:

 

1. Expose students to broad areas of knowledge

c. Read accurately and critically by asking pertinent questions about a text, by recognizing assumptions and implications, and by evaluating ideas. Understand the fundamental concepts, principles, and theories of the natural sciences

 

2. Utilize interpretive reasoning, strategic reasoning, and adaptive reasoning in all academic subjects.

 

3. Demonstrate a sense of harmonious interconnectedness between self, others, nature, and God, which exists throughout and beyond time and space

 

4. Encourage the attitudes and practices of healthful living

b. Understand how individual decision making and behavior impact personal health.

 

Methods of instruction:

Lectures:         You are expected to attend all lectures (Addresses GenEd objectives 1)

Reading:          You are expected to read the reading assignments from the text. (Addresses GenEd objectives 1c.)

Labs:               You are expected to attend and participate in every lab – no lab may be made up.  You are expected to take lab quizzes and turn in laboratory reports. (Addresses GenEd objectives 1, 1c, 2, 4, 4b)

Examination:   Regular exams are scheduled to measure your learning. (Addresses GenEd objective 2, 3, 4, and 4b)

Quizzes:          Quizzes may be given at the end of the lecture period to encourage classroom learning over that day’s material.  (Addresses GenEd objectives 2, 3, 4, and 4b)

 

Homework:     Reading homework over the chapters is available on D2L and is DUE the day AFTER we finish covering that chapter in lecture. (Addresses GenEd objectives 2, 3, 4, and 4b)

 

Cheating:        Cheating of any kind will not be tolerated and may result in an F for the test/assignment/or course and possible dismissal from the class.  Don't do it!

 

Attendance:    Attendance is required on a regular basis in lecture and in lab.  The University policy, as stated in the Bulletin, allows one absence for each hour of credit.  Absences in excess of this limit are grounds for dismissal from the class.  It is the student's responsibility to catch up on any material that is missed due to an absence.  Due to the nature of the labs, there will be no make-up labs once the labs in a given week have been completed.  Absences on test days will be excused only for very serious reasons.  If an absence occurs on a test day, the instructor MUST be notified prior to test time.

 

Letter grades:  A = 90% and above

B = 80% - 89%

C = 70% - 79%

D = 60% - 69%

F = 59% and below

 

Grading:         In-class            =   75%

                        Lab                  =   25%

                        Total:                  100%

 

                        Grading in the lab:      Quizzes                                   6%

                                                            Reports                                   7%

                                                            Midterm lab test                      3.5%   

                                                            Final lab test                            3.5%

                                                            Unknown identification          5%   

                                                                                      Total:            25% of overall class grade

 

Lab Quizzes:  Quizzes will be given during the first part of lab after lab introduction.  They will cover the current and previous lab.  If you arrive late and miss the quiz and will receive a zero.

 

Lab Reports:   Questions and sketches are located at the end of each lab exercise.  This constitutes the entirety of what is required of you for the lab “report.”  You are to fill these out as much as possible during lab.  You may have to wait to fill out the rest of the report next week.  Lab reports are due TWO weeks after that lab exercise is started and should be torn out, stapled together and handed in.  DO NOT COPY answers from each other – do your own thinking.

 

 

 

 

Weather:         You may check the following for official delay or closing announcements:

SWAU switchboard (817) 645-3921    KJCR    88.3FM    KXAS TV   NBC 5.  Announcements are typically made between 9 and 10:30pm.  If it is not determined until early morning that classes may be delayed or cancelled (or will continue as usual) the announcement will be made by 6am.

 

Disabilities:     If you have a disability that may require accommodations to complete the requirements of this course please do the following:  1.  Contact the Director of Students with Disabilities and Academic Support, Dorie Cruz, Scales Hall, at ext. 6041 so that she may verify that you are eligible for accommodations.  2.  Please make Dorie Cruz aware of your situation before the end of the 2nd week of classes so that she might be able to assist you better.  The University complies with the provisions set forth in Section 504 and the ADA Act of 1990.

 

 

 

LECTURE SCHEDULE

                                                Required Reading:

August            27        Wednesday     Chapter 1 Introduction

            29        Friday              Chapter 2 Chemistry

September       1          Monday           Chapter 2 Chemistry

3              Wednesday     Chapter 3 Tools of the Lab    

5          Friday              Chapter 3 Tools of the Lab

8          Monday           Chapter 4 Prokaryotes

10        Wednesday     Chapter 4 & 5 Eukaryotes

12        Friday              Chapter 6 Viruses

15        Monday           TEST # 1

17        Wednesday     Chapter 7 Nutrition, Ecology and Growth

19        Friday              Chapter 7

22        Monday           Chapter 8 Metabolism

24        Wednesday     Chapter 8

26        Friday              Chapter 9 Genetics

                        29        Monday           Chapter 9

                        October           1          Wednesday     TEST # 2

                                                3          Friday              FALL BREAK

                                                6          Monday           Chapters 11& 12 Microbial Control

8          Wednesday     Chapter 13   Microbe-Human Interaction

                                                10        Friday              Chapter 13 & 14

                                                13        Monday           Chapter 14 Innate Immunity

15        Wednesday     Chapter 15 Acquired Immunity

17        Friday              Chapter 15 & 16 Immune Disorders

20        Monday           Chapter 16

22        Wednesday     Chapter 17 Diagnosing Infections

24        Friday              TEST # 3

27        Monday           Chapter 18 Cocci

            29        Wednesday     Chapter 18

                        31        Friday              Chapter 19 Gram + Bacilli

November       3          Monday           Chapter 20 Gram - Bacilli

5          Wednesday     Chapter 20

7          Friday              Chapter 21 Miscellaneous Bacteria

10        Monday           Chapter 21

12        Wednesday     TEST # 4

14        Friday              Chapter 22 Fungi

17        Monday           Chapter 23  Parasites

20        Wednesday     Chapter 23

21        Friday              Chapter 24 DNA viruses

24        Monday           Chapter 24

26        Wednesday     THANKSGIVING

            28        Friday              THANKSGIVING

December        1          Monday           Chapter 25  RNA viruses

3          Wednesday     Chapter 25

5          Friday              Chapter 26 Environmental & Applied Microbiology

8          Monday           FINAL EXAM    NOON

 

 

Lab Schedule:

                                                                                  Lab chapter numbers:

 

            Lab 1…………Sept 3 & 4……………………………………………….xiii – xviii, 1

                       

            Lab 2…………Sept 10 & 11…………………………………………….7, 14, 16

                        7-Smear Prep and Simple Staining, 14-Aseptic Techniques, 16-Streak Plates

 

            Lab 3…………Sept 17 & 18…………………………………………….8, 10

                        8-Gram Stain, 10-Spore Stain

 

            Lab 4…………Sept 24 & 25……………………………………………..9, 22, 23, 26

                        9-Acid Fast Stain, 22-Starch Hydrolysis, 23-Lipid Hydrolysis, 26-Casein Hydrolysis

 

            Lab 5…………Oct 1 & 2…………………………………………..Mid-Term Lab Exam

 

            Lab 6…………Oct 8 & 10………………………………………………...20, 27

                        20-Carbohydrate Fermentation, 27-Gelatin Hydrolysis

 

            Lab 7…………Oct 15 & 16……………………………………………….24, 25, 28

                        24-SIM, 25-IMViC tests, 28-Catalase

 

            Lab 8…………Oct 22 & 23………………………………………………..21, 30, 31

                        21-TSI, 30-Oxidase Test, 31-Urea Test

 

            Lab 9…………Oct 29 & 30….....………………………………………….37, 38 Unknowns

            Lab 10………  Nov 5 & 6…………….……………………………………38 Unknowns

            Lab 11………  Nov 12 & 13……………………………………………… 38 Unknowns

 

            Lab 12………  Nov 19 & 20………………………………………………..44, 53

44-Handwashing, 53-Microbes from the Human Body

 

            Lab 13 ……… Nov 26 & 27……………………………………….THANKSGIVING BREAK 

            Lab 14 ……….Dec 3 & 4…………………………………………..FINAL LAB EXAM

 

 

 

 

 

Microbiology lab rules:

 

1.                  Students are NOT to be in the laboratories without supervision

 

2.                  NO food or beverages allowed in lab.

 

3.                  Bunsen burners are real fire!  Long hair must be tied back throughout the lab exercises.  Always verify that gas to the burner is completely shut off when finished.

 

4.                  Never leave an experiment unattended.

 

5.                  Immediately notify lab instructors in case of a cut or spill.  Spills must be cleaned with a germicidal cleanser.  Cuts must be washed with hydrogen peroxide.

 

6.                  Anything that has come in contact with bacteria must be sterilized and must NOT go in a common waste basket.

 

7.                  Appropriate footwear must be worn at all times.  Sandals and open-toed shoes are not acceptable.  High heeled shoes are not appropriate.

 

8.                  Clothing appropriate for laboratory safety must be worn. Cover legs and arms as much as possible.  Tank tops, halters, short shorts, cutoffs, etc. are not acceptable.  If a student does not comply with this standard, a lab coat must be worn, and buttoned.

 

9.                  Jewelry should not be worn to avoid contamination.

 

10.              Sweep up broken glass, do not pick it up with your hands and dispose in marked glassware box.

 

11.              FIRST thing you do in lab is clean your work space.

 

12.              LAST thing you do in lab is clean your work space.

 

13.              Always remove tape from test tubes when you are finished with them.

 

14.              Cell phones should be off and beepers on silent

 

15.              NO books or notebooks except your lab manual – to prevent contamination to your books

 

16.              Wash hands with germicidal soap before and after each lab period

 

17.              Clothes and hands can become stained – plan ahead and don’t wear your best!

 

18.              Other rules may be added at instructor’s discretion.